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Oxidative phosphorylation does not regulate hypoxic stabilization of HIF-1α A) Cells deficient in SURF-1 (null cells) and cells reconstituted with a SURF-1 cDNA (wild-type) were adapted to glucose or galactose media and cell death was measured after 72 hr. Ectopic expression of PGC-1α using adenoviral infection of differentiated C2C12 cells led to increased levels of electron transport chain proteins, such as cytochrome c, as well as a greater than 2-fold increase in mitochondrial number and oxygen consumption compared with cells infected with CELL METABOLISM : JUNE 2005 green fluorescent protein (GFP) (Figures 3A and 3B).
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